Oxidative stress leads to the formation of esterified erythro- and threo-dihydroxy-fatty acids in HepG2 cells.

ABSTRACT: Oxidative stress plays a central role in pathophysiology. To assess oxidative stress, sensitive methods are required to monitor the cellular damage caused by reactive oxygen species. Phospholipid-bound isoprostanes, such as 5-iPF_2α-VI determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) are currently the best markers for oxidative stress. Here, we describe erythro- and threo-dihydroxy-polyunsaturated fatty acids (PUFA), the hydrolysis products of trans- and cis-epoxy-PUFA, as new biomarkers of oxidative stress in cells. This is demonstrated in four oxidative stress models in HepG2 cells using radical-forming tert-butyl hydroperoxide, glutathione peroxidase 4 inhibiting RSL-3, the redox cycling agent paraquat or rotenone blocking the electron transport chain. L

SUBMITTER: Scholz L 

PROVIDER: S-EPMC11964660 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature