Cryo-EM structures of mouse bestrophin 1 channel in closed and partially open conformations.

ABSTRACT: Bestrophin 1 (BEST1) channels are calcium-activated Cl^- channels involved in diverse physiological processes, including gliotransmitter release in astrocytes. Although human and chicken BEST1 orthologs have been extensively studied, the structural and functional properties of mouse BEST1 (mBEST1) remain poorly understood. In this study, we characterized the structure-function of mBEST1-BF, a C-terminally tagged variant, using whole-cell patch-clamp recordings, surface biotinylation assays, and single-particle cryo-electron microscopy. Cryo-electron microscopy structural analysis of mBEST1-BF revealed closed and partially open conformations. Comparative analysis with human and chicken BEST1 orthologs highlighted conserved calcium-binding and gating mechanisms, with distinct featu

SUBMITTER: Kim KW 

PROVIDER: S-EPMC11964753 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature