Biotransformation of quercetin by Bacillus subtilis and anticancer activity evaluation: in vitro and in Silico.

ABSTRACT: Biotransformation of quercetin by Bacillus subtilis ATCC 23,857 yielded a major metabolite; apigenin-7-O-pentoside (A7P) and 9 other phenolic metabolites. They were elucidated by LC-ESI-TOF-MS/MS analysis. Furthermore, the cytotoxic potential of the biotransformed products was compared to their parent substrate (S) using MTT assay against 2 cancer cell lines; A549 and Caco-2. After 72 h. incubation, the BPs showed concentration-dependent cytotoxic activity against Caco-2 and A549 with IC₅₀ equal to 0.32 and 0.78 mg/ml, respectively. Both BPs and S were safe on the normal human skin fibroblast (hFB) cell line. Molecular docking confirmed the high binding affinity of A7P towards phosphatidyl inositide 3-kinase delta (PI3Kδ) and Cathepsin B protein (CatB) expressed in lung and colo

SUBMITTER: El Gendy SN 

PROVIDER: S-EPMC11965081 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature