Identification of Cell Fate Determining Transcription Factors for Generating Brain Endothelial Cells.

ABSTRACT: Reliable models of the blood-brain barrier (BBB), wherein brain microvascular endothelial cells (BMECs) play a key role in maintenance of barrier function, are essential tools for developing therapeutics and disease modeling. Recent studies explored generating BMEC-like cells from human pluripotent stem cells (hPSCs) by mimicking brain-microenvironment signals or genetic reprogramming. However, due to the lack of comprehensive transcriptional studies, the exact cellular identity of most of these cells remains poorly defined. In this study we aimed to identify the most likely master transcription factors (TFs) for inducing brain endothelial cell (EC) fate and assess the transcriptomic changes following their introduction into immature ECs. Therefore, we first generated PSC-derived immature

SUBMITTER: Ramezankhani R 

PROVIDER: S-EPMC11965213 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature