TGF-β Enhances Phosphate-Driven Calcification of Human OA Articular Chondrocytes.

ABSTRACT: The pathological relevance of articular cartilage calcification in osteoarthritis (OA) is becoming increasingly evident. We are only beginning to understand the pathobiological mechanisms that contribute to articular cartilage calcification in OA. How molecular environmental factors interact with calcification mechanisms is poorly explored. In this study, we developed an in vitro phosphate-driven calcification model for human OA articular chondrocytes, in which these cells are cultured in the presence of calcification medium containing adenosine triphosphate (ATP) and β-glycerophosphate (BGP). We employed this model to investigate the role of transforming growth factor β (TGF-β) in chondrocyte calcification. Chondrocyte culture in calcification medium resulted in mineral nodule formation o

SUBMITTER: Stassen RHMJ 

PROVIDER: S-EPMC11965260 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature