Abundant clock proteins point to missing molecular regulation in the plant circadian clock.

ABSTRACT: Understanding the biochemistry behind whole-organism traits such as flowering time is a longstanding challenge, where mathematical models are critical. Very few models of plant gene circuits use the absolute units required for comparison to biochemical data. We refactor two detailed models of the plant circadian clock from relative to absolute units. Using absolute RNA quantification, a simple model predicted abundant clock protein levels in Arabidopsis thaliana, up to 100,000 proteins per cell. NanoLUC reporter protein fusions validated the predicted levels of clock proteins in vivo. Recalibrating the detailed models to these protein levels estimated their DNA-binding dissociation constants (K_d). We estimate the same K_d from multiple results in vitro, extending the m

SUBMITTER: Urquiza-Garcia U 

PROVIDER: S-EPMC11965494 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature