Association between root canals and gingival sulci microbiota in secondary and persistent endodontic infections.

ABSTRACT: Secondary/persistent endodontic infections (SPEIs) result from failed root canal therapy, causing persistent apical periodontitis. Current diagnostic methods for SPEIs predominantly rely on clinical and radiographic indicators, which often lack adequate sensitivity and specificity. Consequently, there is an urgent need to effectively detect SPEIs or monitor their progression. The aim of this study was to compare and characterize the microbiota of root canals and gingival sulci of teeth affected by SPEI to identify keystone pathogens as potential diagnostic biomarkers through advanced next-generation sequencing (NGS) techniques. Ninety samples from 30 affected teeth in 25 patients undergoing nonsurgical retreatment were analyzed. Bacterial DNA was extracted, the V3-V4 region of the 16S rRNA

SUBMITTER: Park DH 

PROVIDER: S-EPMC11965569 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature