Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction.

ABSTRACT:

Introduction

Evidence demonstrates that sensory neurons respond to pathogenic/allergic infiltration and mediate immune responses, forming an integral part of host defense that becomes hypersensitized during allergy. Our objective was to investigate how asthmatic induction alters the pulmonary neuroimmune transcriptome. We hypothesized that asthmatic induction would upregulate genes in the vagal ganglia (nodose/jugular ganglia), which would be associated with asthmatic immunity, and that these would be clustered, primarily in nodose neurons. Furthermore, lungs would increase transcripts associated with nerve activation, and these would be centered in neural and neuroendocrine-like cells.

Methods

Standard RNA sequencing, single nucleus-RNA sequencing, and spatial RNA sequencin

SUBMITTER: McSwiggin H 

PROVIDER: S-EPMC11965707 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature