Selection of the Most Suitable Culture Medium for Patient-Derived Lung Cancer Organoids.

ABSTRACT:

Introduction

Patient-derived organoids have emerged as a promising in vitro model for precision medicine, particularly in cancer, but also in noncancer-related diseases. However, the optimal culture medium for culturing patient-derived lung organoids has not yet been agreed upon. This study aimed to shed light on the optimal selection of a culture media for developing studies using patient-derived lung organoids.

Methods

Tumor and normal paired tissue from 71 resected non-small cell lung cancer patients were processed for organoid culture. Lung cancer organoids (LCOs) were derived from tumor tissue and normal lung organoids (LNOs) from nonneoplastic lung tissue. Three different culture media were compared: permissive culture medium (PCM), limited culture medium (LCM), and mi

SUBMITTER: Acosta-Plasencia M 

PROVIDER: S-EPMC11965841 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature