Greener and Whiter Analytical Procedure for Theobromine and Caffeine Determination in Tea Using Dimethyl Carbonate as an Extraction Solvent and Mobile Phase Constituent in Reversed-Phase Liquid Chromatography.

ABSTRACT: For the first time, dimethyl carbonate was applied as the sole organic solvent, both as an eluent in solid-phase extraction (SPE) and for the simultaneous determination of theobromine and caffeine in tea extracts using reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC). The proposed procedure has been validated and complies with the requirements of green analytical chemistry (GAC). Solid-phase extraction was used to purify the samples, achieving high recovery rates (97-101%) and precision (RSD < 2%). The method showed satisfactory sensitivity (achieving limit of quantitation values of 0.2 ng for theobromine and 0.35 ng for caffeine), although it was poorer compared to the developed conventional HPLC method using methanol, where the values reached 0.045 and 0.05 ng, respe

SUBMITTER: Kalisz O 

PROVIDER: S-EPMC11966322 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature