Development of a lytic Ralstonia phage cocktail and evaluation of its control efficacy against tobacco bacterial wilt.

ABSTRACT:

Introduction

Bacterial wilt (BW) caused by Ralstonia pseudosolanacearum is a devastating soil-borne disease. Bacteriophages are important biocontrol resources that rapidly and specifically lyse host bacteria, showing good application potential in agricultural production.

Methods

This study isolated nine phages (YL1-YL9) and, using host range and pot experiments, identified two broader host range phages (YL1 and YL4) and two higher control efficacy phages (YL2 and YL3), which were combined to obtain five cocktails (BPC-1-BPC-5).

Results

Pot experiments showed that BPC-1 (YL3 and YL4) had the highest control efficacy (99.25%). Biological characterization revealed that these four phages had substantial thermal stability and pH tolerance. Whole genome sequencing an

SUBMITTER: He H 

PROVIDER: S-EPMC11966396 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature