Treatment of Acute Liver Injury through Selective Tropism of <i>High Mobility Group Box 1</i> Gene-Silenced Large Peritoneal Macrophages.

ABSTRACT: Tissue-resident macrophages (TRMs) are attractive cells to therapeutically deliver oligonucleotide and other gene-expression modifying modalities to treat a wide array of diseases ranging from inflammatory to autoimmune, and even cancer. Here, we focus on TRMs located inside the peritoneal cavity lining the abdomen that selectively express a transcription factor GATA6 called large peritoneal macrophages (GLPMs) and successfully demonstrate functional GLPM-selective delivery of a Cy5-fluorophore-labeled siRNA encapsulated in C12-200 cationic-lipidoid-based nanoparticles (siRNA-Cy5 (C12-200)). Despite being TRMs, GLPMs possess a specific migratory ability to peritoneally located liver tissue upon injury incited by acetaminophen (APAP) overdose in mice. A rapid, liver injury-driven tropism of

SUBMITTER: Oza D 

PROVIDER: S-EPMC11966748 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature