Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors.

ABSTRACT:

Background

Bacteria employ diverse molecular systems, such as the type VI secretion system (T6SS) to outcompete other microorganisms and adapt to ecological niches. The T6SS is a versatile nanomachine capable of delivering toxic effectors into neighboring cells, providing advantages in bacterial interactions. In recent years, T6SSs have been proposed as promising tools for engineering selective antimicrobial platforms.

Results

In this study, we successfully engineered Pseudomonas putida KT2440 to heterologously express and release T6SS effectors. The expression of Tse1, an effector from Pseudomonas chlororaphis, induced sporulation in plant-beneficial Bacillus strains via a T6SS-dependent mechanism, particularly when Tse1 was paired with a PAAR protein. Similarly, the engine

SUBMITTER: Perez-Lorente AI 

PROVIDER: S-EPMC11966926 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature