Single-nucleus RNA sequencing reveals ARHGAP28 expression of podocytes as a biomarker in human diabetic nephropathy.

ABSTRACT:

Introduction

Diabetic kidney disease (DKD) represents serious diabetes-associated complications, and podocyte loss is an important histologic sign of DKD. The cellular and molecular profiles of podocytes in DKD have yet to be fully elucidated.

Methods

This study analyzed kidney-related single-nucleus RNA-seq datasets (GSE131882, GSE121862, and GSE141115) and human diabetic kidney glomeruli transcriptome profiling (GSE30122). ARHGAP28 expression was validated by western blot and immunohistochemistry.

Results

In human kidney tissues, 154 differentially expressed genes (DEGs) were identified in podocytes, which were enriched in biological processes related to nephron development and extracellular matrix-receptor interactions. Similarly, in the mouse kidney, 344 DEGs were

SUBMITTER: Zhang F 

PROVIDER: S-EPMC11967489 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature