Engineering Caffeic Acid O-Methyltransferase for Efficient De Novo Ferulic Acid Synthesis.

ABSTRACT: Ferulic acid is a high-value chemical synthesized in plants. The ferulic acid biosynthesis is still affected by the insufficient methylation activity of caffeic acid O-methyltransferase (COMT). In this study, we engineered COMT from Arabidopsis thaliana to match caffeic acid, and the mutant COMT ^{N129V-H313A-F174L} showed 4.19-fold enhanced catalytic efficiency for degrading caffeic acid. Then, we constructed the de novo synthesis pathway of ferulic acid by introducing tyrosine ammonia lyase from Flavobacterium johnsoniae (FjTAL), 4-hydroxyphenylacetate 3-hydroxylase from Escherichia coli (EcHpaBC), and mutant COMT ^{N129V-H313A-F174L}, and further increased tyrosine synthesis. Furthermore, we overexpressed two

SUBMITTER: Shang HQ 

PROVIDER: S-EPMC11968058 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature