Immunosuppression of spleen in mice treated with erythropoietin: transcriptomic and immunological analysis.

ABSTRACT:

Background and aim

Long term high-dose erythropoietin (EPO) had been reported inducing the formation of abdominal aortic aneurysm (AAA) in mice. When using this model, we found that EPO treated mice showed significant splenomegaly. This is an interesting phenomenon, and its mechanism has not been reported. Therefore, this study aims to explore its mechanism.

Methods

C57BL/6 mice were given intraperitoneal injection of recombinant human EPO at 10000 IU/kg/day, and the control mice were treated with normal saline (vehicle). After 3 weeks, the spleens were harvested. Pathological changes in histology were observed using Hematoxylin and Eosin (H&E) staining. The differential expression genes (DEGs) were identified using RNA sequencing (RNA-Seq), verified with the real-time quant

SUBMITTER: Lyu X 

PROVIDER: S-EPMC11968373 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature