Proteomes and ubiquitylomes reveal the regulation mechanism of cold tolerance mediated by <i>OsGRF4</i> in rice.

ABSTRACT: Low temperature is one of the major abiotic stresses that severely restrict the development of rice. It has been demonstrated previously that OsGRF4 enhances cold tolerance in rice, the molecular mechanism of which remains unknown. This study employed a combination of proteome and ubiquitylome approaches to analyze OsGRF4 mediated chilling between the overexpression line (OX) and wild type (CK). Proteome results showed that 6,157 proteins were identified and 5,045 proteins were quantified after 24-h cold treatment. A total of 59 proteins were upregulated and 63 proteins were downregulated in the OX24 vs. OX0 group; 27 proteins were upregulated and 34 proteins were downregulated in OX24 vs. CK24. Finally, 3,789 ubiquitination modification sites were located on 1,846 proteins,

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11968423 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature