The role and function validation of P2RX4 as a novel cancer biomarker in pan-cancer analysis.

ABSTRACT: Purinergic Receptor P2X4 (P2RX4) is implicated in the carcinogenesis of several cancers, but no extensive study on its role in different forms of cancer. Expression level, gene mutation, immune infiltration, pathway enrichment, and prognostic value analysis of P2RX4 were performed based on multiple publicly available databases such as TCGA, GTEx, GEO, TIMER2, cBioportal, and Metascape databases. Western blot and RT-qPCR were used to identify P2RX4 expression in liver hepatocellular carcinoma (LIHC) and paracancer samples. P2RX4 was knocked in glioblastoma cell line (U251) and prostate cancer cell line (PC3), and its effects on cell viability, apoptosis, migration and invasion were investigated through cell counting kit-8 assay, flow cytometry, wound healing and trasnwell assays, respective

SUBMITTER: Qiao X 

PROVIDER: S-EPMC11968906 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature