Improved production of thermostable pectinase from novel Bacillus siamensis (BIOSMNF45) using computational-conventional approach.

ABSTRACT: The demand for thermostable pectinase in the global commercial market has expanded progressively at a rapid pace. Many industrial processes are operated at elevated temperatures to break down plant polysaccharides (pectin) owing to the significant use of thermotolerant pectinase. Therefore, the study aimed to optimize the medium constituents for enhanced pectinase production from a thermotolerant pectinolytic bacteria (BIOSMNF45) in combination with the one-variable-at-a-time (OVAT) and response surface methodology (RSM). The isolated BIOSMNF45 with high thermostable pectinase activity was identified as Bacillus siamensis by 16S rRNA sequencing. OVAT technique was used in the preliminary screening of different variables (incubation time, incubation temperature, pH, carbon%, organic nitroge

SUBMITTER: Satpathy A 

PROVIDER: S-EPMC11968981 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature