Application of HEMA-AAm copolymer to achieve faster optical tissue transparency for 2D/3D fluorescence imaging.

ABSTRACT: Optical transparency methods can facilitate biological tissue optical imaging, which enabled accurate three-dimensional (3D) signal visualization and quantification of complex biological structures. Unfortunately, existing optical clearing approaches present a compromise between maximizing clearing capability, the preservation of fluorescent protein emission and the speed of sample processing. To address this challenge, we synthesis of a 2-hydroxyethyl methacrylate (HEMA)-acrylamide (AAm) copolymer using antipyrine (ATP) and 2,2'-thiodiethanol (TDE) as solvent, which could embed tissue samples rapidly and highly transparent, and compatible with multiple fluorescence labeling. It can enable volumetric imaging of tissue up to the scale of mice organs, shrinkage duration of the clearing and p

SUBMITTER: Ma H 

PROVIDER: S-EPMC11969017 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature