Conditional Overexpression of Net1 Enhances the Trans-Differentiation of Lgr5⁺ Progenitors into Hair Cells in the Neonatal Mouse Cochlea.

ABSTRACT: Sensorineural hearing loss is mainly caused by damage to hair cells (HC), which cannot be regenerated spontaneously in adult mammals once damaged. Cochlear Lgr5⁺ progenitors are characterised by HC regeneration capacity in neonatal mice, and we previously screened several new genes that might induce HC regeneration from Lgr5⁺ progenitors. Net1, a guanine nucleotide exchange factor, is one of the screened new genes and is particularly active in cancer cells and is involved in cell proliferation and differentiation. Here, to explore in vivo roles of Net1 in HC regeneration, Net1 ^loxp/loxp mice were constructed and crossed with Lgr5 ^CreER/+ mice to conditionally overexpress (cOE) Net1 in cochlear Lgr5⁺ progenitors. We observed a large num

SUBMITTER: Lin Y 

PROVIDER: S-EPMC11969244 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature