Relaxation Optimized Heteronuclear Experiments for Extending the Size Limit of RNA Nuclear Magnetic Resonance.

ABSTRACT: The application of NMR to large RNAs has been limited by the inability to perform heteronuclear correlation experiments essential for resolving overlapping ¹H NMR signals, determining interproton distance restraints and interhelical orientations for structure calculations, and evaluating conformational dynamics. Approaches exploiting ¹H-¹³C correlations that are routinely applied to proteins and small RNAs of ∼60 nucleotides or fewer are impractical for larger RNAs due to rapid dipolar relaxation of protons by their attached carbons. Here we report a ²H-enhanced, ¹H-¹⁵N correlation approach that enables atom-specific NMR characterization of much larger RNAs. Purine H8 transverse relaxation rates are reduced ∼20-fold with rib

SUBMITTER: Shah A 

PROVIDER: S-EPMC11969551 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature