A High-Throughput Ancient DNA Extraction Method for Large-Scale Sample Screening.

ABSTRACT: Large-scale DNA screening of palaeontological and archaeological collections remains a limiting and costly factor for ancient DNA studies. Several DNA extraction protocols are routinely used in ancient DNA laboratories and have even been automated on robotic platforms. Robots offer a solution for high-throughput screening but the costs, as well as necessity for trained technicians and engineers, can be prohibitive for some laboratories. Here, we present a high-throughput alternative to robot-based ancient DNA extraction using a 96-column plate. When compared to routine single MinElute columns, we retrieved highly similar endogenous DNA contents, an important metric in ancient DNA screening. Mitogenomes with a coverage depth greater than 0.1× could be generated and allowed for taxonomic ass

SUBMITTER: Gilardet A 

PROVIDER: S-EPMC11969639 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature