Multilocus sequence typing of clinical and colonizing isolates of Acinetobacter baumannii and comparison with the world isolates.

ABSTRACT:

Objectives

To establish the epidemiological correlation between the study isolates, Indian and World isolates of Acinetobacter baumannii by performing Multilocus Sequence typing.

Materials and methods

A total of 181 isolates (Sputum (n = 116), lower respiratory tract other than sputum (n = 36), upper respiratory tract (n = 20), Environment (medical) (n = 4), and Blood (n = 5) of Acinetobacter baumannii were retrieved from our repository. DNA was isolated and Multilocus Sequence Typing was performed according to the Pasteur scheme. The amplified fragments were sequenced by outsourcing, and the locus and the sequence types were determined as given in the PUBMLST site. The clonal complexes were assigned using eBURST.

Results and conclusion

Of the 181 isolates, 20 were co

SUBMITTER: Choudhary J 

PROVIDER: S-EPMC11969935 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature