Comparative performance evaluation of bisulfite- and enzyme-based DNA conversion methods.

ABSTRACT:

Background

Bisulfite conversion (BC) has been the gold standard in DNA methylation profiling for decades. During this chemical process, non-methylated cytosines are converted into uracils, while methylated cytosines remain intact. Despite its popularity, BC has major drawbacks when used for sensitive applications with low-quality and -quantity DNA samples, such as the required large amount of DNA input, the caused DNA fragmentation and loss, and the resulting reduced sequence complexity. Lately, to account for BC-related disadvantages the first commercial enzymatic conversion (EC) kit was launched. While EC follows the same conversion principle as BC it uses two enzymatic steps instead of one chemical step with BC. In this study, we validated and compared the conversion performance

SUBMITTER: Simons RB 

PROVIDER: S-EPMC11969950 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature