Immune-featured stromal niches associate with response to neoadjuvant immunotherapy in oral squamous cell carcinoma.

ABSTRACT: Tumor stromal cells (TSCs) play a crucial yet underexplored role in the tumor microenvironment (TME). This study uses single-cell sequencing and spatial transcriptomics on paired tumor specimens from 22 patients with oral squamous cell carcinoma (OSCC) enrolled in a randomized two-arm phase 2 trial, receiving neoadjuvant anti-PD-1 mono-immunotherapy or anti-PD-1 plus docetaxel-cisplatin-5-fluorouracil (TPF) immunochemotherapy. Single-cell analysis reveals increased TSCs within the TME of responders in immunochemotherapy. Notably, significant post-treatment upregulation of SELP⁺ high endothelial venules (HEVs) and APOD⁺ myofibroblastic cancer-associated fibroblasts (myCAFs), alongside a decline in STMN1⁺ capillary endothelial cells (cECs), is specific to the

SUBMITTER: Liu YT 

PROVIDER: S-EPMC11970382 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature