Topological importance of CD8⁺ T-cell enrichment in the tumor microenvironment of classic Hodgkin lymphoma.

ABSTRACT: Classic Hodgkin lymphoma (CHL) histologically consists of Hodgkin Reed-Sternberg (HRS) cells and the tumor microenvironment (TME), but the relationship between TME characteristics and clinical features of CHL remains unclear. We aimed to investigate the effects of the TME structure on the outcomes of patients with CHL. We performed a high-throughput analysis of HRS cells and their topological relationship with the reactive immune cells in the TME. After multiplexed immunofluorescence labeling against CD4, CD8, CD30, CD68, CD163, PD-1, and PD-L1, visual images were analyzed. Phenotypes were assigned to all reactive cells, such as CD4⁺ and CD8⁺ T-cells and macrophages. Since the densities of PD1⁺/CD4⁺ T-cells, CD8⁺ T-cells, and PD-L1

SUBMITTER: Takahashi H 

PROVIDER: S-EPMC11971151 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature