Streamlined gram-scale natural N-glycan production using reversible tagging after oxidative release of natural glycans.

ABSTRACT: The study of natural glycans is fundamental to modern glycomics; however, the functional analysis of these glycans is limited by their low natural abundance, structural heterogeneity, and the absence of efficient preparative-scale isolation methods. Here, we present a streamlined approach for the gram-scale production of complex natural N-glycans in their reducing form by combining oxidative release of natural glycans (ORNG) using household bleach, an innovative cleavable tag chemistry, and optimized multi-dimensional chromatography. Our ORNG process releases N-glycans from kilogram-scale of natural sources containing various glycoproteins, which can be efficiently attached with a 4-aminobenzoate tag, facilitating the selective separation of these glycans from other biomolecules. The tagge

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC11971423 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature