The inflammasome adaptor pycard is essential for immunity against Mycobacterium marinum infection in adult zebrafish.

ABSTRACT: Inflammasomes regulate the host response to intracellular pathogens including mycobacteria. We have previously shown that the course of Mycobacterium marinum infection in adult zebrafish (Danio rerio) mimics the course of tuberculosis in human. To investigate the role of the inflammasome adaptor pycard in zebrafish M. marinum infection, we produced two zebrafish knockout mutant lines for the pycard gene with CRISPR/Cas9 mutagenesis. Although the zebrafish larvae lacking pycard developed normally and had unaltered resistance against M. marinum, the loss of pycard led to impaired survival and increased bacterial burden in the adult zebrafish. Based on histology, immune cell aggregates, granulomas, were larger in pycard-deficient fish than in wild-type controls. Transcriptome analysis with RN

SUBMITTER: Uusi-Makela M 

PROVIDER: S-EPMC11972081 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature