Bioactive compound extraction from giloy leaves and steam using ultrasound: bioactivity, antimicrobial, and LC-MS/MS study.

ABSTRACT: Giloy (Tinospora cordifolia) is a medicinal plant rich in bioactive compounds known for their diverse health benefits. This study examined the nutritional value and biological activity of giloy stems and leaves. Hence, giloy stem (GSE), and leaf extract (GLE) was extracted using green extraction technology, ultrasound, and screening secondary metabolites and bioactive compounds. Using a central composite rotatable design combined with RSM, GSE, and GLE's antioxidant activity, total phenolic content and yield were optimized for solvent-to-solid ratio, sonication time, and solvent concertation. The optimum extraction conditions were found to be 22.5:1, 40 min, and 75%. Additionally, the extract inhibited the growth of S. aureus and E. coli. Screening of optimized extract

SUBMITTER: Singh SM 

PROVIDER: S-EPMC11972245 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature