Genetic Deletion of Galectin-3 Inhibits Pancreatic Cancer Progression and Enhances the Efficacy of Immunotherapy.

ABSTRACT:

Background & aims

Pancreatic ductal adenocarcinoma (PDAC) has a desmoplastic tumor stroma and immunosuppressive microenvironment. Galectin-3 (GAL3) is enriched in PDAC, highly expressed by cancer cells and myeloid cells. However, the functional roles of GAL3 in the PDAC microenvironment remain elusive.

Methods

We generated a novel transgenic mouse model (LSL-Kras^G12D/+;Trp53^loxP/loxP;Pdx1-Cre;Lgals3^-/- [KPPC;Lgals3^-/-]) that allows the genetic depletion of GAL3 from both cancer cells and myeloid cells in spontaneous PDAC formation. Single-cell RNA-sequencing analysis was used to identify the alterations in the tumor microenvironment upon GAL3 depletion. We investigated both the cancer cell-intrinsic function and immunosuppressive f

SUBMITTER: Yang D 

PROVIDER: S-EPMC11972442 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature