Active regulatory elements recruit cohesin to establish cell specific chromatin domains.

ABSTRACT: As the 3D structure of the genome is analysed at ever increasing resolution it is clear that there is considerable variation in the 3D chromatin architecture across different cell types. It has been proposed that this may, in part, be due to increased recruitment of cohesin to activated cis-elements (enhancers and promoters) leading to cell-type specific loop extrusion underlying the formation of new sub-TADs. Here we show that cohesin correlates well with the presence of active enhancers and that this varies in an allele-specific manner with the presence or absence of polymorphic enhancers which vary from one individual to another. Using the alpha globin cluster as a model, we show that when all enhancers are removed, peaks of cohesin disappear from these regions and the erythroid specifi

SUBMITTER: Georgiades E 

PROVIDER: S-EPMC11973168 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature