Establishment and application of multiplex PCR method for detection of <i>Trichophyton verrucosum</i>, <i>Microsporum canis</i>, and <i>Trichophyton mentagrophytes</i> from cattle.

ABSTRACT:

Introduction

Dermatophytosis, which is a contagious fungal skin infection common in animals and humans, is the most common skin disease in cattle. It has a serious negative impact on the livestock industry. In order to circumvent the shortcomings of traditional detection methods such as time-consuming and low isolation rate. Therefore, this study established a simple, rapid and effective diagnostic method to accurately diagnose and differentiate the causative fungi of dermatophytosis, which is of great significance to enhance the prevention and treatment of dermatophytosis in beef cattle farms.

Methods

Three pairs of specific primers were designed using Primer Premier 5.0 from Trichophyton verrucosum, Microsporum canis and Trichophyton mentagrophytes. A

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11973286 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature