Surface display of Lys0859, a <i>Streptococcus suis</i> prophage lysin, on <i>Bacillus subtilis</i> spores and its antibacterial activity against <i>Streptococcus suis</i>.

ABSTRACT:

Introduction

Streptococcus suis, an important zoonotic and opportunistic pathogen in pigs, brings huge economic losses to the pig-raising industry and infects humans with diseases. Phage lysin is regarded as a promising substitute for antibiotics due to its ability to quickly and efficiently kill bacteria without easily developing resistance. However, their clinical applications have been hindered by inherent instability under environmental stressors.

Methods

We constructed B. subtilis spores displaying bacteriophage lysin Lys0859 using spore coat protein CotG as an anchoring motif. Environmental tolerance was evaluated through thermal (37-95°C), pH (1.0-8.0), and enzymatic challenges, while antibacterial efficacy against S. suis was assessed using agar

SUBMITTER: Wang L 

PROVIDER: S-EPMC11973311 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature