Cellular heterogeneity in metabolism and associated microbiome of a non-model phytoflagellate.

ABSTRACT: Single-cell transcriptomics is a key tool for unravelling metabolism and tissue diversity in model organisms. Its potential for elucidating the ecological roles of microeukaryotes, especially non-model ones, remains largely unexplored. This study employed the Smart-seq2 protocol on Ochromonas triangulata, a microeukaryote lacking a reference genome, showcasing how transcriptional states align with two distinct growth phases: a fast-growing phase and a slow-growing phase. Besides the two expected expression clusters, each corresponding to either growth phase, a third transcriptional state was identified across both growth phases. Metabolic mapping revealed a boost of photosynthetic activity in the fast growth over the slow growth stage, as well as downregulation trend in pathways associated

SUBMITTER: Jeevannavar A 

PROVIDER: S-EPMC11973420 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature