Biogeographical distribution of gut microbiome composition and function is partially recapitulated by fecal transplantation into germ-free mice.

ABSTRACT: Fecal microbiota transplantation has been vital for establishing whether host phenotypes can be conferred through the microbiome. However, whether the existing microbial ecology along the mouse gastrointestinal tract can be recapitulated in germ-free mice colonized with stool remains unknown. We first identified microbes and their predicted functions specific to each of six intestinal regions in three cohorts of specific pathogen-free mice spanning two facilities. Of these region-specific microbes, the health-linked genus Akkermansia was consistently enriched in the lumen of the small intestine compared to the colon. Predictive functional modeling on 16S rRNA gene amplicon sequencing data recapitulated in shotgun sequencing data revealed increased microbial central metabolism, lipolytic fe

SUBMITTER: Yang JC 

PROVIDER: S-EPMC11973428 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature