<i>In vitro</i>, <i>in vivo</i>, and cellular mechanisms of <i>Astragalus onobrychis L.</i> extract against protoscoleces and hydatid cysts of <i>Echinococcus granulosus</i>.

ABSTRACT:

Introduction

In this study, we evaluated the in vitro, ex vivo, and in vivo effects of the chloroform extract of Astragalus onobrychis L. (Fabaceae family) (AOCE) on apoptosis induction and DNA damage in protoscoleces and hydatid cysts of Echinococcus granulosus.

Methods

The protoscolicidal properties of AOCE were examined through both in vitro and ex vivo studies on hydatid cyst protoscoleces, utilizing the eosin exclusion assay. Additionally, we evaluated the effects of AOCE on apoptosis induction and DNA damage in the protoscoleces using a colorimetric protease assay and real-time PCR analysis, respectively. The in vivo efficacy was determined by measuring the quantity, dimensions, and mass of hydatid cysts in infected muri

SUBMITTER: Ghasemian Yadegari J 

PROVIDER: S-EPMC11973518 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature