Flagellin engineering enhances CAR-T cell function by reshaping tumor microenvironment in solid tumors.

ABSTRACT:

Background

Adoptive cell therapy using genetically engineered chimeric antigen receptor (CAR)-T cells is a new type of immunotherapy that directs T cells to target cancer specifically. Although CAR-T therapy has achieved significant clinical efficacy in treating hematologic malignancies, its therapeutic benefit in solid tumors is impeded by the immunosuppressive tumor microenvironment (TME). Therefore, we sought to remodel the TME by activating tumor-infiltrating immune cells to enhance the antitumor function of CAR-T cells.

Methods

We engineered CAR-T cells expressing Salmonella flagellin (Fla), a ligand for toll-like receptor 5, to activate immune cells and reshape the TME in solid tumors. Functional validation of the novel Fla-engineered CAR-T cells was performed i

SUBMITTER: Niu X 

PROVIDER: S-EPMC11973770 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature