Supercharged NK cells, unlike primary activated NK cells, effectively target ovarian cancer cells irrespective of MHC-class I expression.

ABSTRACT:

Objective

To demonstrate the significance of supercharged natural killer (sNK) cells to target aggressive gynecological tumours.

Methods and analysis

We used cell cultures of peripheral blood-derived mononuclear cells (PBMCs) and purified NK cells alone and in the presence of tumours. MHC-class gene expression assessments of ovarian tumours were performed using gene set enrichment analysis (GSEA). Secretion and expression levels of cytokines in PBMCs and NK cells were determined using ELISA and scRNA seq analysis, respectively. A flow cytometer was used for surface marker analysis. ⁵¹Cr and eSight were used to determine the killing activity of NK cells.

Results

We have observed a significant decrease in the numbers and functions of NK cells in patients with

SUBMITTER: Huerta-Yepez S 

PROVIDER: S-EPMC11973776 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature