Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis.

ABSTRACT:

Background

Sepsis-induced immunosuppression involves complex molecular mechanisms, including dysregulated long noncoding RNAs (lncRNAs), which remain poorly understood.

Objective

We aimed to identify immunosuppression-related lncRNAs and their functional pathways in sepsis. Methods: Using weighted gene coexpression network analysis (WGCNA), we analyzed lncRNA profiles from peripheral blood mononuclear cells (PBMCs) of three sepsis patients and three healthy controls. Key modules linked to immunosuppression were validated via RT-PCR and external datasets. Pathway enrichment and protein interaction networks were employed to prioritize mechanisms.

Results

A sepsis-associated module containing 4,193 lncRNAs revealed three immunosuppression-related pathways: Th17 cell diff

SUBMITTER: Zhang W 

PROVIDER: S-EPMC11974007 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature