Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens.

ABSTRACT:

Background

Tyrosine is a prerequisite for melanin biosynthesis and plays a crucial role in the growth and development of melanocytes, but the underlying mechanism is still unclear. In our previous research, we added 10^- 9-10^- 6 mol/L tyrosine to the melanocytes of black-bone chickens and found that 10^- 6 mol/L tyrosine significantly increased the tyrosinase content in melanocytes.

Methods

In this study, melanocytes from Xichuan black-bone chickens were used as research objects, 10^- 6 mol/L tyrosine was added for transcriptome sequencing. By analyzing miRNA and mRNA expression profiles, the miRNA-mRNA network was constructed, the targeting relationship was demonstrated by double luciferase reporting experiments, and the influence of

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11974055 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature