Double-negative T cells in combination with ursodeoxycholic acid ameliorates immune-mediated cholangitis in mice.

ABSTRACT:

Background

Primary biliary cholangitis (PBC) is a liver-specific autoimmune disease. Treatment of PBC with ursodeoxycholic acid (UDCA) is not sufficient to prevent disease progression. Our previous study revealed that the number of hepatic double-negative T cells (DNT), which are unique regulatory T cells, was reduced in PBC patients. However, whether replenishment of DNT can prevent the progression of PBC remains unclear.

Methods

DnTGFβRII (Tg) mice and 2OA-BSA-immunized mice received DNT alone or in combination with oral UDCA. After 6-12 weeks of treatment, these mice were assessed for serological changes, liver pathological manifestations and intrahepatic immune responses.

Results

Adoptive transfer of DNT alone significantly decreased serum levels of alanine transa

SUBMITTER: Zhang C 

PROVIDER: S-EPMC11974204 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature