A TRPV4-dependent calcium signaling axis governs lamellipodial actin architecture to promote cell migration.

ABSTRACT: Cell migration is crucial for development and tissue homeostasis, while its dysregulation leads to severe pathologies. Cell migration is driven by the extension of actin-based lamellipodia protrusions, powered by actin polymerization, which is tightly regulated by signaling pathways, including Rho GTPases and Ca²⁺ signaling. While the importance of Ca²⁺ signaling in lamellipodia protrusions has been established, the molecular mechanisms linking Ca²⁺ to lamellipodia assembly are unknown. Here, we identify a novel Ca²⁺ signaling axis involving the mechano-gated channel TRPV4, which regulates lamellipodia protrusions in various cell types. Using Ca²⁺ and FRET imaging, we demonstrate that TRPV4-mediated Ca²⁺ influx upregulates R

SUBMITTER: Iu E 

PROVIDER: S-EPMC11974816 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature