Recovering high-resolution information using energy filtering in MicroED.

ABSTRACT: Inelastic scattering poses a significant challenge in electron crystallography by elevating background noise and broadening Bragg peaks, thereby reducing the overall signal-to-noise ratio. This is particularly detrimental to data quality in structural biology, and the diffraction signal is relatively weak. These effects are aggravated even further by the decay of the diffracted intensities as result of accumulated radiation damage, and rapidly fading high-resolution information can disappear beneath the noise. Loss of high-resolution reflections can partly be mitigated using energy filtering, which removes inelastically scattered electrons and improves data quality and resolution. Here, we systematically compared unfiltered and energy-filtered MicroED data from proteinase K crystals, first

SUBMITTER: Clabbers MTB 

PROVIDER: S-EPMC11974828 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature