Time-lapse tryptic digestion: a proteomic approach to improve sequence coverage of extracellular matrix proteins.

ABSTRACT: The extracellular matrix (ECM) is a complex and dynamic meshwork of proteins providing structural support to cells. It also provides biochemical signals governing cellular processes, including proliferation, adhesion, and migration. Alterations of ECM structure and/or composition have been linked to many pathological processes, including cancer and fibrosis. Over the past decade, mass-spectrometry-based proteomics has become the state-of-the-art method to profile the protein composition of ECMs. However, existing methods do not fully capture the broad dynamic range of protein abundances in the ECM. They also do not permit to achieve the high coverage needed to gain finer biochemical on ECM proteoforms (e.g., isoforms, post-translational modifications) and topographical information c

SUBMITTER: Lee F 

PROVIDER: S-EPMC11974830 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature