A factor-based analysis of individual human microglia uncovers regulators of an Alzheimer-related transcriptional signature.

ABSTRACT: Human microglial heterogeneity is only beginning to be appreciated at the molecular level. Here, we present a large, single-cell atlas of expression signatures from 441,088 live microglia broadly sampled across a diverse set of brain regions and neurodegenerative and neuroinflammatory diseases obtained from 161 donors sampled at autopsy or during a neurosurgical procedure. Using single-cell hierarchical Poisson factorization (scHPF), we derived a 23-factor model for continuous gene expression signatures across microglia which capture specific biological processes (e.g., metabolism, phagocytosis, antigen presentation, inflammatory signaling, disease-associated states). Using external datasets, we evaluated the aspects of microglial phenotypes that are encapsulated in various in vitro

SUBMITTER: Marshe VS 

PROVIDER: S-EPMC11974870 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature