Quantitative Analysis of Coupled Carbon and Energy Metabolism for Lignin Carbon Utilization in <i>Pseudomonas putida</i>.

ABSTRACT: Soil Pseudomonas species, which can thrive on lignin-derived phenolic compounds, are widely explored for biotechnology applications. Yet, there is limited understanding of how the native metabolism coordinates phenolic carbon processing with cofactor generation. Here, we achieve quantitative understanding of this metabolic balance through a multi-omics investigation of Pseudomonas putida KT2440 grown on four common phenolic substrates: ferulate, p-coumarate, vanillate, and 4-hydroxybenzoate. Relative to succinate as a non-aromatic reference, proteomics data reveal >140-fold increase in proteins for transport and initial catabolism of each phenolic substrate, but metabolomics profiling reveals that bottleneck nodes in initial phenolic compound catabolism maintain more f

SUBMITTER: Zhou N 

PROVIDER: S-EPMC11974891 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature