Valosin-containing protein p97 extracts capping protein CP110 from the mother centriole to promote ciliogenesis.

ABSTRACT: The primary cilium is a crucial signaling organelle that can be generated by most human cells, and impediments to primary ciliogenesis lead to a variety of developmental disorders known as ciliopathies. The removal of the capping protein, CP110, from the mother centriole is a key early step that promotes generation of the ciliary vesicle and ciliogenesis. Recent studies have demonstrated that CP110 undergoes polyubiquitination and degradation in the proteosome, but the mechanisms of unfolding and removal from the mother centriole remain unknown. Herein we demonstrate that p97/Valosin-containing protein (VCP or Cdc48), a member of the ATPase Associated with diverse Activities (AAA) protein family, is responsible for removal of CP110 from the mother centriole. We show that use of p97 knockdo

SUBMITTER: Dewanji R 

PROVIDER: S-EPMC11974959 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature