Assessing gut microbial provisioning of essential amino acids to host in a murine model with reconstituted gut microbiomes.

ABSTRACT: Gut microbial essential amino acid (EAA) provisioning to mammalian hosts remains a critical yet poorly understood aspect of host-microbe nutritional interactions, with significant implications for human and animal health. To investigate microbial EAA contributions in mice with reconstituted gut microbiomes, we analyzed stable carbon isotopes (¹³C) of six EAAs across multiple organs. Germ-free (GF) mice fed a high-protein diet (18%) were compared to conventionalized (CVZ) mice fed a low-protein diet (10%) following fecal microbiota transplantation 30 days prior and a 20-day dietary intervention. We found no evidence for microbial EAA contributions to host tissues, with ¹³C-EAA fingerprinting revealing nearly identical patterns between GF and CVZ organs. Both groups mai

SUBMITTER: Ayayee P 

PROVIDER: S-EPMC11975013 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature