Chemical genomics informs antibiotic and essential gene function in Acinetobacter baumannii.

ABSTRACT: The Gram-negative pathogen, Acinetobacter baumannii, poses a serious threat to human health due to its role in nosocomial infections that are resistant to treatment with current antibiotics. Despite this, our understanding of fundamental A. baumannii biology remains limited, as many essential genes have not been experimentally characterized. These essential genes are critical for bacterial survival and, thus, represent promising targets for drug discovery. Here, we systematically probe the function of essential genes by screening a CRISPR interference knockdown library against a diverse panel of chemical inhibitors, including antibiotics. We find that most essential genes show chemical-gene interactions, allowing insights into both inhibitor and gene function. For instance, knockdown of li

SUBMITTER: Tran JS 

PROVIDER: S-EPMC11975115 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature